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Antimicrobial Testing of Marine Biosurfactant

Compared with chemically produced surfactants, marine biosurfactants (MBS) have many advantages, such as high biodegradability and low ecotoxicity, and can be easily produced from renewable energy sources. These microbially derived surface-active substances are widely used in the pharmaceutical, food, cosmetic, textile, petroleum and agricultural industries. They are useful as antifungal and antibiofilm agents. Given the rise in antibiotic resistance, it has become clear that new antimicrobial agents need to be identified and a way to fix the antibiotics currently used in medicine. There is a global call for arms in international efforts to tackle the challenge of antibiotic resistance. MBS are well suited to answer the call for their applications including: bactericidal, bacteriostatic, biofilm formation inhibition, biofilm disruption, synergy and adjunctive action with antibiotics. For example, rhamnolipids may be more effective against gram-positive bacteria than gram-negative bacteria because of the presence of an outer membrane in gram-negative bacteria that can exclude biosurfactant molecules.

Fig. 1 Schematic representation of biosurfactants with antimicrobial activity produced by microorganisms associated with human health according to the human body districts.Fig. 1 Schematic representation of biosurfactants with antimicrobial activity produced by microorganisms associated with human health according to the human body districts. (De Giani A, et al., 2021)

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Given the rise in antibiotic resistance, it is a major obstacle to the development of antimicrobials. It results in an expensive, time-consuming and often unsuccessful development process. Our R&D platform can be successfully applied to the development of a broad range of highly biodegradable, low ecotoxicity surfactants, cost-effectively and shorten the time to clinical stage.

  • Topical Antimicrobial Testing of MBS
    When testing MBS, microbiological and preclinical testing is recommended. This will not only validate their efficacy, but also address safety and labelling claims.
  • Cytotoxicity Assay of MBS
    The cytotoxicity of crude MBS will be assessed in eukaryotic cells by lactate dehydrogenase (LDH) release and total cell number assay. At CD BioSciences, all experiments will be repeated three times in triplicate each time.
  • In-Vitro Time-kill Assay of MBS
    CD BioSciences offers test methods to determine the time kill or bacterial activity rate of MBS. We help our customers test their products or product dilutions by exposing them to aerobic and anaerobic microorganisms.
  • Zone of Inhibition
    This highly controlled method tests products for their ability to inhibit bacterial growth and can identify key MBS properties. When the clear area of the sample was examined, this indicated no bacterial growth.
  • Antimicrobial Efficacy Testing of MBS
    • Virucidal efficacy testing
    • Fungicidal efficacy testing
    • Tuberculocidal efficacy testing
    • Sporicidal efficacy testing
Antimicrobial Testing at CD BioSciences

Antimicrobial Testing at CD BioSciences

  • CD BioSciences can help with all your antimicrobial testing needs. From project consulting for MBS testing to meeting quality standards and regulations, we can keep your business compliant and maintain purity.
  • CD BioSciences has an extensive library of organisms in-house to meet your testing needs. CD BioSciences' biobanks include methicillin-resistant S. aureus, vancomycin-resistant enterococci (VRE), and commercially available isolates.

CD BioSciences is a professional service provider for the marine biology industry. Our antimicrobial testing of MBS service can ensure that the most suitable methods and techniques are selected for your project. We provide our customers with the most precise ingredient data and highly informed process expertise. Our team of biomass experts plays a key role in the formulation, optimization and commercial evaluation of biomass value-added processes in industry and academia. If necessary, please feel free to contact us.


  • De Giani A, Zampolli J, Di Gennaro P. Recent trends on biosurfactants with antimicrobial activity produced by bacteria associated with human health: Different perspectives on their properties, challenges, and potential applications[J]. Frontiers in microbiology, 2021, 12: 655150.

Please kindly note that our services can only be used to support research purposes (Not for clinical use).

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