Lemna Growth Inhibition Test

The Lemna growth inhibition test is a standardized method for assessing the phytotoxicity of chemicals and environmental samples using the duckweed species Lemna minor. Lemna is a representative species of higher aquatic plants. These plants are small, easy to grow, and sensitive to chemicals. Plant protection products that act as herbicides or plant growth regulators need to be tested on duckweed. The Lemna test is also considered a suitable alternative to the dark matter algae test. The test involves exposing Lemna plants to a range of concentrations of a test substance and monitoring the plants for growth inhibition over a specified period (usually 7-10 days). The testing is performed in a controlled laboratory environment where environmental conditions such as temperature, light, and nutrient availability are carefully managed. If your product shows considerable toxicity, you may want to do a duckweed restoration study or test it with other higher aquatic plants such as foxtail or glycerin.

Our Services

Our team of experts has extensive experience in conducting the Lemna growth inhibition test and is proficient in the testing protocols and regulatory requirements.

• Single substance testing
  We evaluate the harmful effects of water-soluble substances and wastewater on the growth of the plants by analyzing different parameters such as leaf number, leaf area, chlorophyll content, and fresh or dry weight.
• Testing of waste water
  The Lemna growth inhibition test is also suitable for testing wastewater samples, especially for dark and/or fluorescent samples.
• Recovery studies
  If aquatic plants are determined to be at high risk based on standard duckweed testing, recovery studies may help provide further information on the extent of the impact of the test item on higher aquatic plants.

Course of Lemna Growth Inhibition Test

• Depending on the properties of your substance, duckweed studies can be performed in a static test design without updating the test items.
• For precipitating or degrading substances, a semi-static test design with renewable test items is appropriate. Typically, a dose-response study is performed.
• The duration of exposure was 7 days, with assessments typically performed on days 3 and 5. Leaf number will be counted and dry weight determined at the end of the test.
• Test substance concentrations were analyzed at the beginning of the test and at the end of the test to verify the dose of the test item. Toxicity references tested twice a year demonstrate the sensitivity and applicability of the test system.

Results Delivery

At CD ComputaBio, we provide our clients with clear, concise, and timely reports detailing the test parameters, data analysis methods, and test results.

We deliver the results in a format that is easy to understand and can be used to make informed decisions about the safety and risks of various substances.

We also provide consultation services to help our clients interpret the results of the test and understand their implications fully.

CD BioSciences is a professional service provider for the marine biology industry. Our Lemna growth inhibition test services can ensure that the most suitable methods and techniques are selected for your project. We provide our customers with the most precise ingredient data and highly informed process expertise. Our team of biomass experts plays a key role in the formulation, optimization and commercial evaluation of biomass value-added processes in industry and academia. If necessary, please feel free to contact us.

Please kindly note that our services can only be used to support research purposes (Not for clinical use).