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Identification of Microcystins

Microcystins are an increasingly important group of bioactive compounds produced by many major planktonic cyanobacteria. Microcystins are cyclic heptapeptide cyanobacterial toxins produced by members of several cyanobacterial genera including Microcystis, Planktothrix (Oscillatoria), Anabaena, Nostoc, Anabaenopsis, Hapalosiphon. They are a family of cyclic heptapeptides that cause acute and chronic toxicity. Purified microcystins are used in a range of research applications, including toxicological and biochemical studies, the development of detection systems, and the study of water treatment strategies. Microcystin-LR is the most toxic form of the more than 80 known toxic variants and the most studied family form. Commercial availability of purified microcystins remains relatively limited, and for many projects, the cost of purchase is prohibitive. At CD BioSciences, we offer detection assays that include immunological or biochemical assays such as enzyme-linked immunosorbent assay and enzyme activity assays, chemical methods such as high-performance liquid chromatography and more sophisticated liquid chromatography–mass spectrometry.

General structure of microcystins.Fig. 1 General structure of microcystins. (McElhiney J, et al., 2005)

Our Services

CD BioSciences provides isolation and identification services for microcystins using our state-of-the-art equipment. Our analytical services support the full custom detection workflow. Our services are listed as folloes, but are not limited to:

  • Biochemical Screening Services
    • Mouse assays
    • Enzyme linked immunosorbent assay (ELISA)
    • Protein phosphatase inhibition assay
    • Polyclonal antibody methods
    • Monoclonal antibody methods
    • Recombinant antibody fragment methods
    • Molecularly imprinted polymers
    • Brine shrimp assay
  • Physicochemical Identification Services
    • Liquid chromatography–mass spectrometric (LC–MS) detection
    • Electrochemical detection
    • Capillary electrophoresis (CE) detection
    • Gas chromatographic (GC) and GC–MS analysis

Our Analysis Platform

  • Bioanalytical Development
    Our scientists build repeatable analyses, coordinate requests, and generate bioanalytical insights on a unified digital platform.
  • Bioprocess Development
    Proven biologics process development workflows are derived from industry best practices and built on a unified digital platform.

Why Use Our Isolation and Identification Services of Microcystins?

Our Isolation and Identification Services of Microcystins
  • Scientific team with extensive experience in handling marine biotoxin
  • Fully customizable designs to meet each specific need
  • Our LC-MS analysis service provides a sufficiently reliable and inexpensive analysis for the routine detection of toxins.
  • Advanced platform to ensure optimal results for every step
  • Fast turnaround time and reasonable price
  • Reliable and up-to-date analysis reports
  • Friendly, expert advice available

CD BioSciences is a professional service provider for the marine biology industry. Our identification services of microcystins can ensure that the most suitable methods and techniques are selected for your project. We provide our customers with the most precise ingredient data and highly informed process expertise. Our team of biomass experts plays a key role in the formulation, optimization and commercial evaluation of biomass value-added processes in industry and academia. If necessary, please feel free to contact us.

Reference

  • McElhiney J, Lawton L A. Detection of the cyanobacterial hepatotoxins microcystins[J]. Toxicology and applied pharmacology, 2005, 203(3): 219-230.

Please kindly note that our services can only be used to support research purposes (Not for clinical use).

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