Marine Microbial Viability Testing

Marine microorganisms are considered as potential sources of novel enzymes because they are relatively more stable than corresponding enzymes from plants and animals. Enzymes from marine microbes also differ from their homologous enzymes in terrestrial microbes based on salinity, pressure, temperature, and light conditions. Marine microorganisms and their enzymes, which are active under high salt concentrations, wide temperature ranges, high light and pressure incidence, are an important source of unique biocatalysts. Marine microbial enzymes are useful in various industrial applications. The stability of marine microbial enzymes is closely related to the vitality and metabolic capacity of marine microorganisms. Marine microbial viability assays are often used as a proxy for assessing the overall metabolism of marine microorganisms. The diversity of these assays and the little information provided by some assay kits or online protocols often lead to errors or poor interpretation of results. Furthermore, the use of some of these assays is limited to simple systems and care must be taken when applying them to environmental samples. CD BioSciences has a mature and proven verification system to measure the viability of marine microorganisms.

Our Services

Our laboratory work begins in the R&D phase: different approaches and parameters are explored and tested on a small scale to identify successful approaches. Depending on customer requirements, successfully developed analysis programs can be further validated. Viability analysis will be performed on the client's marine microbial samples after the analytical program has been successfully established.

• Marine microbiology media test kit
• Offshore waters and legionella management
• Microbial corrosion and biocide treatment
• Culture-based testing
  Culture-based assays for marine bacterial viability include conventional colony counts and related methods.
• PCR-based testing
  Microbial viability is assessed using a polymerase chain reaction (PCR)-based strategy that has been developed to distinguish nucleic acids associated with living cells from those associated with nonviable cells.

Highlights of Our Testing Technology of Viability PCR

Traditional methods of measuring marine microbial viability rely on time-consuming culturing, colony counting or microscopy. Additionally, certain marine organisms cannot be cultured with current technology. Therefore, these viable but unculturable species require molecular viability measurements. Viability PCR is a reliable and culture-free method for the sensitive and rapid detection of viable microorganisms.

• Sensitive and rapid viability analysis without time-consuming culture, microscopy or colony counting
• Allows for marine strain-specific viability analysis
• Validated and published against dozens of marine bacterial, marine fungal and marine viral species
• Compatible with next-generation sequencing applications

Workflow of Our Marine Microbial Viability Testing

Although a given project has special characteristics, it can often be approached by a rigid approach, divided into distinct phases: (1) A research and development phase, (2) An optional validation phase, and finally, (3) Analyze samples.

CD BioSciences is a professional service provider for the marine biology industry. Our marine microbial viability test services can ensure that the most suitable methods and techniques are selected for your project. We provide our customers with the most precise ingredient data and highly informed process expertise. Our team of biomass experts plays a key role in the formulation, optimization and commercial evaluation of biomass value-added processes in industry and academia. If necessary, please feel free to contact us.

Reference

• Iliou K, Kikionis S, Ioannou E, et al. Marine Biopolymers as Bioactive Functional Ingredients of Electrospun Nanofibrous Scaffolds for Biomedical Applications[J]. Marine Drugs, 2022, 20(5): 314.

Please kindly note that our services can only be used to support research purposes (Not for clinical use).