Unsaturated Fatty Acids Biosynthesis from Schizochytrium

Schizochytrium is a fungus-like heterotrophic unicellular marine eukaryote that is ubiquitous in coastal marine ecosystems worldwide. They have attracted attention due to their ability to produce high levels of long-chain polyunsaturated fatty acids (LC-PUFAs), especially docosahexaenoic acid (DHA, C22:6n3). Schizochytrium limacinum SR21 has been extensively characterized to produce DHA. The DHA content of total fatty acids in Schizochytrium may approach 40%. During microbial fermentation for the production of lipids and polyunsaturated fatty acids, the generated ROS and reactive aldehydes impair cellular metabolism and inhibit lipid productivity. However, PUFA production in Schizochytrium is attractive because these microorganisms possess the necessary biosynthetic machinery and, unlike field plants, can grow rapidly using inexpensive media under controlled conditions in bioreactors. Fermentation technology allows year-round production. Schizochytrium and other thraustochytrids in unsaturated fatty acids production do not require light. CD BioSciences' staff have many years of experience in marine unsaturated fatty acids biosynthesis process design and optimization. Our labs focus on the efficient delivery of science, compliance, customer service and data. All teams work hard to provide exceptional service, delivering data that fits your schedule.

Fig. 1 Strategies for enhancing the ω-3 LC-PUFA production by fermentation. (Chi G, et al., 2021)

Our Services

CD BioSciences provides high quality unsaturated fatty acid biosynthesis services. Our excellent technology can realize microbial fermentation, process design, yield improvement services, and solve all difficulties encountered in research for customers.

• Unsaturated Fatty acid Biosynthesis in Schizochytrium
  Commercial fermentation requires competent microbial strains. Acceptable strains must have high productivity. CD BioSciences improves productivity by mutagenizing Schizochytrium strains. CD BioSciences utilizes metabolic engineering and genetic engineering tools to modify genes associated with target enzyme and regulatory protein expression to enhance unsaturated fatty acid biosynthesis
• Fermentation Process Design
  Our scientists use possible fermentor operating schemes including batch, fed-batch, repeated fed-batch, and continuous culture.
• Fermentation Process Improvement
  CD BioSciences controls the quality and composition of media and inoculum in fermentation process optimization. Batch and fed-batch operations are often preferred in commercial large-scale fermentations at CD BioSciences.
• Use of chemical modulators
• Coculture and sonication

Our Features of Unsaturated Fatty Acids Biosynthesis from Schizochytrium Services

• The talented scientists at CD BioSciences with extensive experience in metabolic engineering, genetic engineering, transcriptomics can avoid common pitfalls in the design of unsaturated fatty acid biosynthesis in schizochytrium.
• Our experts will work closely with you to assist in the design and optimization of your custom unsaturated fatty acid synthesis and functional properties optimization program.
• We not only directly provide customers with unsaturated fatty acid biosynthesis, but also provide customers with high-quality scientific reports on experimental results in a timely manner, but also provide customers with rich experimental experience and guidance.

CD BioSciences is a professional service provider for the marine biology industry. Our unsaturated fatty acids biosynthesis services can ensure that the most suitable methods and techniques are selected for your project. We provide our customers with the most precise ingredient data and highly informed process expertise. Our team of biomass experts plays a key role in the formulation, optimization and commercial evaluation of biomass value-added processes in industry and academia. If necessary, please feel free to contact us.

Reference

• Chi G, Xu Y, Cao X, et al. Production of polyunsaturated fatty acids by Schizochytrium (Aurantiochytrium) spp[J]. Biotechnology Advances, 2021: 107897.

Please kindly note that our services can only be used to support research purposes (Not for clinical use).