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Marine Halophilic Enzyme Production

Marine halophilic enzymes are enzymes that are produced by microorganisms adapted to live in high-salt environments, such as the ocean or salt lakes. These enzymes have specific characteristics that enable them to function and remain stable in the presence of high salt concentrations. They have similar characteristics to traditional enzymes but differ in properties, mainly structural ones. Halophilic microorganisms are able to survive high salt concentrations because they have developed a variety of biochemical, structural, and physiological modifications that enable the catalytic synthesis of proteins with interesting physicochemical and structural properties. Marine halophilic enzymes can withstand and function at high salt concentrations, which is beneficial for industrial processes involving salt or brine solutions. Marine halophilic enzymes exhibit higher stability in the presence of salt. These enzymes retain their catalytic activity at extreme pH levels, temperature, and pressure, broadening their potential applications. CD BioSciences is a leading provider of marine halophilic enzyme services, committed to delivering high-quality solutions for a wide range of industries. With our expertise in marine enzyme research and development, we offer a comprehensive suite of services tailored to meet the specific needs of our clients.

Our Services

CD BioSciences is your trusted partner in marine halophilic enzyme production services, dedicated to driving innovation and ensuring your success in the ever-evolving landscape of biotechnology.

  • Characterization of Enzymatic Features of Halophilic Bacteria
    We use qualitative enzyme assays to characterize the enzymatic characterization of halophilic bacteria.
  • Qualitative Enzyme Assays
    • Preparation of extracellular crude enzyme
    • Amylase activity
    • Protease activity
    • Glutaminase activity
    • Lipase activity
  • Determination of the Optimal NaCl Concentration, pH, and Temperature for Enzymes' Activities
    • To study the effect of salt on amylase activity, quantitative enzyme assays are performed in reaction buffers containing increasing concentrations of NaCl (0, 0.5, 0.75, 1.0, 1.5, 2.0 and 2.5 M).
    • Determination of enzyme activity in the pH range 6-12 and temperature range 25-60°C.
  • Custom Enzyme Engineering
    To meet unique industrial requirements, we provide customized enzyme engineering services. Leveraging our expertise in protein engineering, we modify enzyme structures and functionalities through techniques such as mutagenesis, directed evolution, and rational design.

Our Methods

Our Methods

Our marine halophilic enzyme services are underpinned by an array of cutting-edge methodologies, ensuring accurate and reliable results:

  • Metagenomic Analysis: By extracting and sequencing the total DNA from diverse saltwater samples, we identify genes encoding halophilic enzymes and their variants for subsequent analysis.
  • Gene Cloning and Expression: At CD BioSciences, we have proficiency in gene cloning and heterologous expression to produce recombinant enzymes at high yields.
  • Protein Purification: Our experts utilize various purification strategies, including affinity chromatography, ion exchange chromatography, and size exclusion chromatography, to isolate and purify recombinant enzymes to homogeneity.
  • Enzyme Assays and Analysis: We employ a wide range of enzyme assays including spectrophotometric assays, HPLC, gel electrophoresis, and mass spectrometry analysis, enabling precise characterization of enzyme properties.

CD BioSciences is a professional service provider for the marine biology industry. Our marine halophilic enzyme production services can ensure that the most suitable methods and techniques are selected for your project. We provide our customers with the most precise ingredient data and highly informed process expertise. Our team of biomass experts plays a key role in the formulation, optimization and commercial evaluation of biomass value-added processes in industry and academia. If necessary, please feel free to contact us.

Please kindly note that our services can only be used to support research purposes (Not for clinical use).

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