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Daphnia sp., Acute Immobilisation Test

Acute toxicity test to evaluate the effects of chemicals on Daphnia sp. Young daphnids, aged less than 24 hours at the start of the test are exposed to a range of concentrations (at least five concentrations) of the test substance for 48 hours. Fixation was recorded at 24 and 48 h and compared to control values.

CD BioSciences is a leading provider of comprehensive acute immobilization testing services using Daphnia sp. Our services encompass the identification and assessment of the impact of different types of chemicals, including plant protection products and pharmaceuticals, on Daphnia sp. Our testing services are designed to identify the susceptibility of organisms to different chemical compounds, thereby providing our clients with the necessary information to make informed decisions.

Our Services

Our testing services are carried out by a team of highly skilled and experienced scientists using state-of-the-art equipment that adheres to regulatory guidelines and internationally recognized protocols. We pride ourselves on our commitment to delivering testing services with high accuracy, reliability, and regulatory compliance.

  • Alternative species testing
  • Acute immobilisation test of Daphnia sp.
  • Daphnia sp. culture
  • Identifying the concentrations that cause a significant reduction of mobility compared to the controls.
  • Preparing comprehensive reports that include test results, discussions, recommendations, and regulatory compliance.


CD BioSciences follows the OECD 202 guideline for acute immobilization testing on Daphnia sp. LOEC, NOEC, and EC50 (EC20, EC10) are determined in the immobility test of the daphnids.

What we offer?

  • Scientific expertise: Our team of experts is highly skilled with years of experience and technical know-how to conduct and interpret acute immobilization testing services on Daphnia sp.
  • Regulatory compliance: Our testing services are compliant with relevant regulatory frameworks.
  • Cost-effectiveness: We offer affordable and competitive pricing solutions for our testing services.
  • Data confidentiality: We ensure that our client's data is secure and confidential throughout the testing process.

Test Organism

The water flea Daphnia magna belongs to the class Crustacea, subclass Phyllopoda. The juvenile Daphnia we use are less than 24 hours old and are cultured in our specialized laboratory. We also provide Chironomus riparius, Ceriodaphnia dubia, Tetrahymena thermophile, Brachionus calyciflorus and Thamnocephalus platyurus. The test organisms are not limited to the listed, please contact us for other organisms.

CD BioSciences is a professional service provider for the marine biology industry. Our Daphnia sp. reproduction test services can ensure that the most suitable methods and techniques are selected for your project. We provide our customers with the most precise ingredient data and highly informed process expertise. Our team of biomass experts plays a key role in the formulation, optimization and commercial evaluation of biomass value-added processes in industry and academia. If necessary, please feel free to contact us.

Please kindly note that our services can only be used to support research purposes (Not for clinical use).

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